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                                      
   	                        B )       "   H  H«  H·  HÁ  J§  M  P1³	Üø hÃ'ÈÉ÷'kÜìø;R	ø	

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äQšµ	¯Ù;,:ÉÏHæñ	n¾Q	°YJSºZ¼Ïdïn,X ×dÎv
æ…Í�žOå¢
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-IAÑ
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‘Ñ  ­*$B½	   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  1<?xml version="1.0"?><Features nextValidID="7"><Feature recentID="0" name="tet promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="196-201" color="#ffffff" type="standard"/><Segment range="202-218" color="#ffffff" type="standard"/><Segment name="-10" range="219-224" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; promoter for tetracycline efflux protein gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="TcR" directionality="1" translationMW="41523.57" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="272-1462" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tet&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to tetracycline&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;tetracycline efflux protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"/></Q></Feature><Feature recentID="2" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1948-2536" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1637-1655" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2985-3071" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2866-2893" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1660-1676" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  Ù<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="M13 Reverse" sequence="CAGGAAACAGCTATGAC" description="In lacZ gene. Also called M13-rev"><BindingSite location="1659-1675" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="1659-1675" bases="CAGGAAACAGCTATGAC"/></BindingSite><BindingSite simplified="1" location="1659-1675" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="1659-1675" bases="CAGGAAACAGCTATGAC"/></BindingSite></Primer><Primer recentID="1" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="2436-2455" boundStrand="0" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="2436-2455" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="2436-2455" boundStrand="0" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="2436-2455" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="2" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="623-642" boundStrand="1" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="623-642" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="623-642" boundStrand="1" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="623-642" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="3" name="T7" sequence="TAATACGACTCACTATAGGG" description="T7 promoter, forward primer"><BindingSite location="1635-1654" boundStrand="1" annealedBases="TAATACGACTCACTATAGGG" meltingTemperature="49"><Component hybridizedRange="1635-1654" bases="TAATACGACTCACTATAGGG"/></BindingSite><BindingSite simplified="1" location="1635-1654" boundStrand="1" annealedBases="TAATACGACTCACTATAGGG" meltingTemperature="49"><Component hybridizedRange="1635-1654" bases="TAATACGACTCACTATAGGG"/></BindingSite></Primer></Primers>
  *<Notes>
<UUID>771aafa0-9923-47fb-996c-5d4fd8770a36</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pNH4</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="3:57:19">2021.5.13</Created>
<LastModified UTC="3:57:19">2021.5.13</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference title="Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting." pubMedID="21493687" authors="Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF" journal="Nucleic Acids Res. 2011 Apr 14. ():." journalName="Nucleic Acids Res. 2011 Apr 14. ():." type="Journal Article"/>
</References>
</Notes>
  Y     –         
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   )<?xml version="1.0"?><CustomEnzymeSets/>
